AVS 46th International Symposium
    Biomaterial Interfaces Group Tuesday Sessions
       Session BI-TuA

Paper BI-TuA4
Characterization of Supported Biomimetic Films Using Broadband Vibrationally Resonant Sum-Frequency Generation

Tuesday, October 26, 1999, 3:00 pm, Room 613/614

Session: Characterization of Biomaterial Interfaces
Presenter: K.A. Briggman, National Institute of Standards and Technology
Authors: K.A. Briggman, National Institute of Standards and Technology
T. Petralli-Mallow, National Institute of Standards and Technology
L.J. Richter, National Institute of Standards and Technology
A.L. Plant, National Institute of Standards and Technology
J.C. Stephenson, National Institute of Standards and Technology
Correspondent: Click to Email
Supported organic films have received considerable attention as model biological membranes, as well as biomolecular templates for the development of biomimetic devices. A complete characterization of these biomimetic films requires the application of in-situ techniques, capable of probing fully hydrated systems. We have been exploring the potential of broadband vibrationally resonant sum frequency generation (SFG) as an in-situ probe for the study of hybrid bilayer membranes (HBMs). Our novel broadband approach@footnote 1@ provides a complete SFG spectrum over a window of several hundred wavenumbers, combining interface sensitivity and molecular specificity with the advantages of short acquisition times and no need for wavelength tuning. We have acquired vibrational SFG spectra of a variety of supported biomolecular compounds, including phospholipids in HBMs. A discussion of the preparation and stability of the HBMs as examined by SFG will be presented. @FootnoteText@ @footnote 1@Vibrationally resolved sum-frequency generation with broad-bandwidth infrared pulses, Opt. Lett. 23 1594 (1998).