AVS 46th International Symposium
    Biomaterial Interfaces Group Monday Sessions
       Session BI-MoA

Paper BI-MoA4
Complement Activation by IgM Immobilized on Methylated Silicon

Monday, October 25, 1999, 3:00 pm, Room 613/614

Session: Protein Solid-Surface Interactions I
Presenter: P. Tengvall, Linkoping University, Sweden
Authors: P. Tengvall, Linkoping University, Sweden
A. Askendal, Linkoping University
K.I. Lundström, Linkoping University, Sweden
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The activation of the complement system by IgM immobilized on methylated silicon was studied by ellipsometry/antibody techniques. The IgM coated surfaces were incubated in normal human- and depleated sera at 37°C up to 90 minutes, and the deposited amounts of characteristic proteins probed by antibodies. The results indicate, surprisingly, that the so prepared model surface activates the human complement in a manner independent of calcium, although via the classical pathway.