AVS 46th International Symposium
    Biomaterial Interfaces Group Monday Sessions
       Session BI-MoA

Paper BI-MoA3
Oriented Protein Binding via Silane-SAMs Using His-tagged Proteins

Monday, October 25, 1999, 2:40 pm, Room 613/614

Session: Protein Solid-Surface Interactions I
Presenter: G.E.M. Tovar, Fraunhofer Institute for Interfacial Engineering and Biotechnology, Germany
Authors: C. Hoffmann, University of Stuttgart, Germany
H. Brunner, Fraunhofer Institute for Interfacial Engineering and Biotechnology, Germany
G.E.M. Tovar, Fraunhofer Institute for Interfacial Engineering and Biotechnology, Germany
Correspondent: Click to Email
Oriented immobilization of proteins is a promising way of retaining protein activity on artificial surfaces.@footnote 1@ Modern protein engineering has already developed suitable tags for this purpose. Surface engineering is now required to provide complementary surfaces. We present experimental data on surface functionalization of silicon oxide surfaces using silane SAMs and the subsequent oriented binding of his-tagged proteins. We synthesized nitrilo-triacetic acid (NTA) silanes for direct attachment to silicon oxide surfaces, and NTA derivatives@footnote 2,3,4@ reactive to surfaces functionalized using epoxy terminated silanes. The stepwise surface functionalization and the binding capacitiy of the NTA surfaces for histidine peptides and his-tagged proteins has been investigated using XPS, MALDI-TOF-MS, contact angle measurements and in situ-ellipsometry. @FootnoteText@ @footnote 1@ Rao, S.V., Anderson, K.W., Bachas, L.G., Mikrochim. Acta, 1998, 128, 127-143. @footnote 2@ Dietrich, C., Schmitt, L., Tampe, R., Proc. Natl. Acad. Sci. USA, 1995, 92, 9014-9018. @footnote 3@ Sigal, G.B., Bamdad, C., Barberis, A., Strominger, J., Whitesides, G.M., Anal. Chem., 1996, 68, 490-497. @footnote 4@ Liley, M., Keller, T.A., Duschl, C., Vogel, H., Langmuir, 1997, 13, 4190-4192.