AVS 46th International Symposium
    Biomaterial Interfaces Group Friday Sessions
       Session BI-FrM

Paper BI-FrM5
Utilizing Direct Communication between Enzymes and Conducting Polymers in Glucose Sensors

Friday, October 29, 1999, 9:40 am, Room 613/614

Session: Interface, Properties, and Modification
Presenter: A. Kros, University of Nijmegen, The Netherlands
Authors: A. Kros, University of Nijmegen, The Netherlands
S.W.F.M. van Hövell, TNO Nutrition and Food Research Institute, The Netherlands
D.M. Vriezema, University of Nijmegen, The Netherlands
R.J.M. Nolte, University of Nijmegen, The Netherlands
Correspondent: Click to Email
Biosensors are currently of great interest because of the potential to measure a variety of substrates like glucose and lactate.@footnote 1@ Glucose is by far the most studied analyte in this field of research, primarely due to its importance in human metabolic processes. Here we report the development of a glucose sensor, which will be utilized to measure in vivo glucose levels in the near future. The working mechanism of the sensor is based upon direct electron communication between the enzyme glucose oxidase and a conducting polymer.@footnote 2@ In this new glucose sensor, ethylenedioxythiophene is polymerized chemically inside the pores of a cyclopore track-etch membrane using iron(III)chloride as a catalyst. In this way, a thin layer of conducting polymer (polyethylenedioxythiophene, pedot) is deposited in the interior of the pores. The latter layer is subsequently covered with the redox enzyme glucose oxidase by means of physical adsorption and electrostatic interactions between the positively charged pedot and the negatively charged enzyme. The resulting sensor is able to detect glucose in the clinical relevant concentration range via amperometric methods. The influence of electrostatic interactions and the use of electronic mediators on the sensor performance will be discussed. @FootnoteText@ @footnote 1@ A.E.G. Cass, A practical approach, Oxford University Press, New York, 1994. @footnote 2@ C.G.J. Koopal, B. de Ruiter, R.J.M. Nolte, J. Chem. Soc. Chem. Commun., 1991, 1691.