AVS 45th International Symposium
    Biomaterial Interfaces Group Tuesday Sessions
       Session BI-TuM

Paper BI-TuM11
Multifunctional Molecular Multilayer Assemblies for Reagentless, Fluorescence-Based Biosensing

Tuesday, November 3, 1998, 11:40 am, Room 326

Session: Biosensor-Biology Interface
Presenter: G.P. Lopez, University of New Mexico
Authors: M.M.A. Sekar, University of New Mexico
P.D. Hampton, University of New Mexico
G.P. Lopez, University of New Mexico
Correspondent: Click to Email
This report describes the development of self-assembled monolayers (SAMs) and surface modified SAMs to create model, multifunctional organic surfaces for reagentless, fluorescence-based biosensing. We describe the formation of modular molecular assemblies that display biospecific ligands, luminescent probes and highly-hydrated oligomeric species that inhibit nonspecific adsorption of biomolecules and cells [e.g., oligo(ethylene glycol)]. SAMs can be formed by the serial chemisorption of amine-terminated alkylthiolates to gold or alkylsilanes to silica, and can be serially modified by reaction with di-aldehydes, and subsequently di-amines, to form layered molecular assemblies that can be terminated with bioreceptive ligands, luminescent probes or oligo(ethylene glycol). This talk will present a detailed surface analytical characterization of these layered molecular assemblies and will discuss the efficacy of this method for constructing modular, multifunctional assemblies for interfacial modification of biomaterials. We demonstrate that protein-sensitive luminescent probes immobilized on such layered assemblies can be used to detect specific and nonspecific adsorption of proteins through steady-state and time-resolved fluorescence spectroscopy.