AVS 45th International Symposium
    Biomaterial Interfaces Group Monday Sessions
       Session BI-MoP

Paper BI-MoP8
Enhancing the Sensitivity and Selectivity of the Solid-Phase Immunoassay Using Water Soluble Polymer Coatings

Monday, November 2, 1998, 5:30 pm, Room Hall A

Session: Biomaterial Interfaces Poster Session
Presenter: M. Natesan, Geocenters, Inc.
Authors: M. Natesan, Geocenters, Inc.
S. Metzger, Geocenters, Inc.
R.J. Colton, Naval Research Laboratory
G.U. Lee, Naval Research Laboratory
Correspondent: Click to Email
Immunoassays have gained acceptance as both a bioanalytical technique and diagnostic tool due to the availability of antibodies from a variety of species against a wide range of analytes. These assays are often conducted on a solid surface to facilitate separation of the enzymes or radioisotopes that are typically used to amplify the antibody-analyte reaction. The antibody is usually immobilized on a hydrophobic surface by spontaneous adsorption, which is a process driven by molecule-surface interactions and/or conformational changes. The complex nature of this spontaneously adsorbed film can lead to erroneous signal due to the unwanted adsorption of assay constituents or antibody conformational changes. In this presentation we will describe two new schemes for directly immobilizing antibodies at a surface using a monolayer of water-soluble polymer that resists adhesion of proteins and cells. One technique uses direct covalent immobilization of the antibody to the polymer film while the other uses a molecular recognition interaction for assembly. We will describe the physical properties of the polymer films and compare the activity of the polymer immobilized antibodies to spontaneously adsorbed antibodies.