AVS 45th International Symposium
    Biomaterial Interfaces Group Monday Sessions
       Session BI-MoM

Invited Paper BI-MoM7
Interfacial Supra-Biomolecular Assemblies on Solid Supports

Monday, November 2, 1998, 10:20 am, Room 326

Session: Protein Solid-Surface Interactions
Presenter: W. Knoll, The Institute of Physical and Chemical Research (RIKEN), Japan
Authors: W. Knoll, The Institute of Physical and Chemical Research (RIKEN), Japan
A. Offenhaeusser, Max-Planck-Institut für Polymerforschung, Germany
Correspondent: Click to Email
This contribution summarizes some of our efforts in designing, preparing, and characterizing supramolecular interfacial assemblies integrating biomolecular functional units. Among the presented bio-interfaces are oligonucleotide matrices fabricated by self-assembly strategies based on thiol coupling to Au-substrates or on biotin-streptavidin interactions. A multispot parallel read-out of hybridization reactions between various surface-bound capture probes and complement strands from solution is presented. The concept is based on surface plasmon microscopy and image analysis computer routines. It is shown how the interfacial architecture can be optimized for maximum binding efficiency by using monomolecular layers assembled from binary thiol solutions composed of the catcher probes and diluent molecules that control the lateral separation of the individual binding sites. First results on the influence of the ionic strength, the degree of mismatch, and the temperature are presented. In addition the extension of this concept to PNA catcher probes in discussed. The second class of surface architectures concerns tethered membranes. Various concepts for the coupling of lipid bilayers to solid supports of different materials (Au, SiOx) based on polymers or peptides are briefly discussed. Particular emphasis is put on the structural analysis of the complex multilayer assembly and on the functional characterization by electrochemical techniques. The reconstitution of membrane-integral ion translocating proteins into the supported bilayers bears great potential for biosensor formats.