Paper NM-TuP1
alginate based Nanocomposite for Microencapsulation of Probiotic: Effect of Cellulose Nanocrystal (CNC) and Lecithin

Tuesday, December 4, 2018, 4:00 pm, Room Naupaka Salon 1-3

Lactobacillus rhamnosus ATCC 9595 were encapsulated in alginate-CNC-lecithin microbeads for the production of nutraceutical microcapsules. The concentration of CNC and lecithin in alginate microbeads was optimized by monitoring the viability of L. rhamnosus after stabilization of the microbeads by freeze-drying. Results showed that alginate microbeads (AC-0) reduced by 0.95 log CFU/g whereas addition of 13 wt% CNC in alginate microbeads (AC-3) reduced (P > 0.05) by 0.01 log CFU/g of bacterial viability after freeze drying. Incorporation of 2 wt% lecithin in alginate-CNC microbeads (ACL-1) also revealed a protective effect during free drying same as alginate-CNC microbeads (AC-3). The compression strength of the freeze-dried ACL-1 microbeads improved 40% compared to alginate microbead alone. Swelling studies revealed that addition of CNC and lecithin in alginate microbead decreased (around 47%) the gastric fluid absorption but increased the dissolution time by 20 min compared to alginate microbeads (A-0). During complete transition through the gastric passage, the viability of L. rhamnosus in dried ACL-1 microbeads was 52% higher as compared to L. rhamnosus encapsulated in A-0 based beads. The viability of L. rhamnosus was also evaluated during storage at 25 and 4˚C. It was found that at 25 and 4˚C storage conditions, the viability of L. rhamnosus encapsulated in ACL-1 microbeads decreased by 1.23 and 1.08 log respectively, whereas the encapsulation with A-0 microbeads exhibited a 3.17 and 1.93 log reduction respectively.