| Pacific Rim Symposium on Surfaces, Coatings and Interfaces (PacSurf 2018) | |
| Biomaterial Surfaces & Interfaces | Wednesday Sessions |
| Session BI-WeE |
| Session: | Biomolecule/Material Interactions and Medical Applications |
| Presenter: | Håkan Nygren, University of Gothenburg, Göteborg, Sweden |
| Authors: | H. Nygren, University of Gothenburg, Göteborg, Sweden C. Zhang, Science for Life Laboratory, Stockholm, Sweden M. Arif, Science for Life Laboratory, Stockholm, Sweden M. Uhlen, Science for Life Laboratory, Stockholm, Sweden |
| Correspondent: | Click to Email |
Bone fractures affect hundreds of millions people worldwide and are a leading cause of long-term pain and disability. Fractured bone normally heals ad integrum through a process undergoing characteristic stages of blood coagulation, inflammation, formation of soft and hard callus and, finally, remodeling to its original structure. In approximately 10% of femur-neck fractures, healing meets with failure, or delay. Common causes of failure to heal are critical size defects, infection, or mobility of the fracture parts. Internal stabilisation of fractures with metal implants is an efficient aid of fracture healing. Tissue engineering of bone healing is efficiently made by implanting metal species like Mg, Sr, Zn and Mn. These metals often have a capacity to catalyze formation of hydroxyapatite in bone tissue (Nygren, Pacsurf 2016) suggesting a possible common pathway for the well documented effect of trace metals on bone healing. In this study we analysed the transcriptomics of fracture healing with and without implanted Mg and Mn after 4 and 7 Days of healing, before mineralization and after completion of the callus bone.
Proteins coded by the most differentially expressed genes during normal fracture healing after 4 Days of healing where regulators of platelet degranulation, upregulators of TGF-beta, regulators of Beta-1 Integrin, IL10 receptor antagonist and ROBO proteins guiding cell movement in embryos.
Proteins most differentially expressed after 7 Days of healing were an enzyme hydrolysing lysine, inhibitors of inflammation, NFkappaB, microtubule associated scaffold protein, angogenic proteins and BMP-2 signalling proteins.
Venn diagrams comparing the up-regulated genes after healing with Mg and Mn after 4 Days of healing showed no overlap between the activated genes in these Groups. After 7 Days of healing, there was an 80% overlap between genes upregulated by Mg and Mn. The data suggest that pathways of bone healing at metal implants differs after 4 Days of healing, before the start of mineralisation, but are more congruent after 7 Days of healing when the callus bone is mineralised and remodelling starts.