| Pacific Rim Symposium on Surfaces, Coatings and Interfaces (PacSurf 2018) | |
| Biomaterial Surfaces & Interfaces | Wednesday Sessions |
| Session BI-WeE |
| Session: | Biomolecule/Material Interactions and Medical Applications |
| Presenter: | Per Malmberg, Chalmers University of Technology, Sweden |
| Authors: | H. Nygren, University of Gothenburg, Göteborg, Sweden C. Zhang, Science for Life Laboratory, Stockholm, Sweden P. Malmberg, Chalmers University of Technology, Sweden |
| Correspondent: | Click to Email |
Metal oxides are widely used in implant materials and trace metals are known to deeply influence bone healing. The present study was undertaken to elucidate the mechanisms of the effect of metal ions on bone healing, starting with analyses of the ability of different metal oxides to catalyze the formation of hydroxyapatite (HA) and ending with a global analysis of the transcriptome of bone tissue after implantation of metal ions.
Incubations of MnO and ZnO with cell culture medium followed by analysis with XPS, ToF-SIMS and SEM/EDX showed that these metal oxides are covered with a layer of HA within 12h. Implantation of MnO and ZnO in rat tibia stimulated the formation of callus bone. After 3w of healing of ZnO implants, the bone mineral contained high levels of Zn. This was considered a potential hazard and the use of ZnO was omitted from the study. Sham-operated tibia and bone implanted with MnO were taken to RNA-extraction and global analysis of differently expressed genes at the Science for Life Laboratory in Stockholm (head M. Uhlen). After 4 days of healing, the enrichment analysis showed upregulation of genes reflecting response to cytokines, cytokine regulation and cytokine production in the bones implanted with MnO, compared to sham. Furthermore, genes reflecting leukocyte migration, inflammation and celldeath were upregulated. Analysis of upregulated single genes shows reactions to hypoxia (RGS5), reactions to platelet Ca levels (LHFPL2), genes related to osteogenesis (FetuinB, RUFY4,NFkBIA) and osteoclast differentiation (CPMB6B). The data are still undergoing further analysis.
Manganese has been described as an essential trace metal for bone formation since the mid 1930´s when low levels of Mn in the feed was shown to cause skeletal defects in chicken, rats and rabbits. Mn has been suggested as a trace metal in bone cement based on its effect on biochemical markers of bone metabolism. Manganese is widely used in biomaterials, most extensively as a component of stainless steel.