| Pacific Rim Symposium on Surfaces, Coatings and Interfaces (PacSurf 2018) | |
| Biomaterial Surfaces & Interfaces | Tuesday Sessions |
| Session BI-TuP |
| Session: | Biomaterial Interfaces Poster Session |
| Presenter: | Jae Young Kim, Daegu Gyeongbuk Institute of Science & Technology |
| Authors: | J.Y. Kim, Daegu Gyeongbuk Institute of Science & Technology S.Y. Lee, Daegu Gyeongbuk Institute of Science & Technology, Korea M.H. Shin, Daegu Gyeongbuk Institute of Science & Technology, Korea D.W. Moon, Daegu Gyeongbuk Institute of Science & Technology, Republic of Korea |
| Correspondent: | Click to Email |
Although atmospheric pressure mass spectrometry (AP-MS) is a promising analytic technique for biological samples because of its ambient analytic process and no or minimal sample pretreatment, still many challenges must be overcome to acquire MS data from them. Because mass spectrometry is a quantitative technique for the measurement of the masses of the charged molecules that comprise a sample of material, small but actual parts should be severed from the sample. Thus, desorption and ionization methods for small spot sampling to the biological sample are the most important technique to minimize the sample damage and to obtain high resolution MS imaging. Currently many ambient desorption/ionization sources except lasers are working in ionized gaseous state or spray state of charged droplet, reducing the size of source devices is limited.
We use electrospray ionization source and visible continuous wave (CW) lasers as AP-sampling/ionization sources and develop/combine these electrospray and lasers for the purpose of mass spectrometry applications. The energetic light generated by CW lasers focuses on a very small spot of the sample through the objective lens. At same time, the electrospray device forms the charged droplet particles on the sample. Regarding desorption and ionization procedures in open air, CW lasers mainly desorb actual parts from the sample and electrospays mainly ionize these small substances.
An inverted optical microscope was used as a sampling stage and the additional pumping system including ion transfer tubing, chamber, and dry pump known as air flow assisted ion transfer equipment was installed with the MS inlet. Therefore an AP-MS system is developed and preliminary MS data are achieved with a help of gold nanoparticles. With an addition of the two-dimensional programmed scanning stage to this ambient MS system, many bio-molecular mass spectrometric imaging were obtained from the mouse hippocampal tissues. High spatial resolution MS imaging with a pixel size of four micrometer can be secured at a sample moving velocity of 30 μm /s. MS imaging of bio-molecules including monoacylglycerols, cholesterols, fragments of sphingolipids and glycerophospholipids has been obtained from mouse hippocampal tissues.