| Pacific Rim Symposium on Surfaces, Coatings and Interfaces (PacSurf 2018) | |
| Biomaterial Surfaces & Interfaces | Tuesday Sessions |
| Session BI-TuP |
| Session: | Biomaterial Interfaces Poster Session |
| Presenter: | Sun Young Lee, Daegu Gyeongbuk Institute of Science & Technology, Korea |
| Authors: | S.Y. Lee, Daegu Gyeongbuk Institute of Science & Technology, Korea H.J. Lim, Daegu Gyeongbuk Institute of Science & Technology J.Y. Kim, Daegu Gyeongbuk Institute of Science & Technology D.W. Moon, Daegu Gyeongbuk Institute of Science & Technology, Republic of Korea |
| Correspondent: | Click to Email |
For last decades, ToF-SIMS has been in use in bio sample imaging and has resulted in some important bio issue discoveries. SIMS analysis requires ultrahigh vacuum environment and analysis in atmospheric not allowed. Its ultra-high vacuum based operation necessitates proper sample preparations ranging from simple ones like washing and drying to relatively sophisticated as frozen hydration.[1] Therefore, any of them can neither represent the biologically native state nor preserve membrane integrity. So we have developed a new sample preparation using wet cells covered by single layer graphene for hydrated and native state bio sample.
Graphene acts as a gas impermeable honeycomb mesh on cells preventing solution evaporation and keeping cells wet even under ultra-high vacuum. Besides, the high electrical conductivity of graphene compensates the charging effects during analysis. ToF-SIMS images in this study were obtained using ToF-SIMS 5 (ION-TOF GmbH) equipped with liquid metal ion gun (LMIG) [2,3] and analyzed both positive and negative ion modes and about 1000 specimen-related spectra were obtained from A549 lung carcinoma cell. Thus, we obtain various lipid SIMS images from cell membrane through a graphene layer without cracking.
Major limitations of applying mass spectrometry imaging techniques such as SIMS to biomedical researches are due to harsh bio-sample preparation including freezing, matrix addition and drying. The possibility that secondary molecular ions can be sputtered through a single layer graphene from wet cells will open innovative applications of mass spectrometry imaging of wet cells to various biomedical research areas.
[1] K. Schaepe, J. Kokesch-Himmelreich, et al, Biointerphases 10.1 (2015): 019016
[2] Vanbellingen, Q. P.; Elie, N.; Eller, M. J.; Della-Negra, S.; Touboul, D.; Brunelle, A. Rapid Commun. Mass Spectrom, 29, 1187-1195 (2015)
[3] Shon, H. K.; Yoon, S.; Moon, J. H.; Lee, T. G. Biointerphases, 11, 02A321 (2016)