| Pacific Rim Symposium on Surfaces, Coatings and Interfaces (PacSurf 2018) | |
| Biomaterial Surfaces & Interfaces | Tuesday Sessions |
| Session BI-TuM |
| Session: | Bioimaging and Bionanotechnology |
| Presenter: | John Fletcher, University of Gothenburg, Göteborg, Sweden |
| Authors: | M. Munem, University of Gothenburg, Göteborg, Sweden K. Dimovska Nilsson, University of Gothenburg, Göteborg, Sweden O. Zaar, Sahlgrenska University Hospital, Gothenburg N. Neittaanmäki, Sahlgrenska University Hospital, Gothenburg J. Paoli, Sahlgrenska University Hospital, Gothenburg J.S. Fletcher, University of Gothenburg, Göteborg, Sweden |
| Correspondent: | Click to Email |
Time-of-fight secondary ion mass spectrometry (ToF-SIMS) is starting to be of increasing value to clinicians and has been used on different tissue samples to successfully identify and localise chemical components to various areas of the tissue and answer disease related questions [1]. Compared to other methods, the main advantage of ToF-SIMS is the label free detection of a large number of different molecules within one experiment on the same tissue section. ToF-SIMS is successfully used for analysing lipids behaviour in biological samples like breast cancer tissue [2]. Basal cell carcinoma (BCC) is one of the most increasing cancers worldwide and it is the most common malignancy in white people. Although the mortality is low as BCC rarely metastasises, this malignancy causes considerable morbidity and places a huge burden on healthcare services worldwide. Furthermore, people who have this condition are at high risk of developing further BCC and other malignancies [3].
Samples were collected from patients with BCC, by Mohs surgery. The tissue was sectioned for ToF-SIMS analysis and H&E staining of consecutive tissue slices was performed. ToF-SIMS was performed using an Ionoptika J105 instrument using a 40 keV (CO2)6000+ ion beam. The analysis provided detailed chemical information about the individual lipid species and the spatial distribution of these within the tissue. It was possible to observe differences between the layers of the skin as well as between healthy and cancerous tissue (see figure). ToF-SIMS data was correlated with H&E stained images to understand and confirm, from which structures or regions of the tissue that the individual signals originated.