| Pacific Rim Symposium on Surfaces, Coatings and Interfaces (PacSurf 2018) | |
| Biomaterial Surfaces & Interfaces | Tuesday Sessions |
| Session BI-TuM |
| Session: | Bioimaging and Bionanotechnology |
| Presenter: | Kajsa Uvdal, Linköping University, Sweden |
| Authors: | A. Skallberg, Linköping University, Sweden K. Bunnfors, Linköping University, Sweden C. Brommesson, Linköping University, Sweden K. Uvdal, Linköping University, Sweden |
| Correspondent: | Click to Email |
Photoelectron Emission Microscopy (PEEM) and Imaging X-ray Photoelectron Spectroscopy (XPS) have the potential to deliver element specific imaging useful for biomedical visualization. This may increase the understanding of biological processes on the cellular level, contributing with element specific information and data on topographical morphology combined. The technique is based on chemical composition, chemical states and work-function shifts.
This is hereby demonstrated by combined PEEM and Imaging XPS investigation of neutrophils and their activation processes. Neutrophils are vital components in the human defense system, with the fundamental role to fight invading pathogens. Neutrophils are also able to release nuclear DNA done by formation of extracellular web-like structures called neutrophil extracellular traps (NETs) to capture and occasionally kill intruding microbes.
Here we reportneutrophils externally triggered by in this case nanoparticle (NPs). The neutrophils and NETs formation are imagined in presence of NPs and we report elemental composition of single-cells and structure of NETs. Active cellular uptake of nanoparticles is imaged both before and after NETs release. Element specific imaging of this novel capability for mass transport. This shows the potential for element specific bio-related cell studies on surfaces and nanoparticle tracking on the cellular level.