| Pacific Rim Symposium on Surfaces, Coatings and Interfaces (PacSurf 2018) | |
| Biomaterial Surfaces & Interfaces | Tuesday Sessions |
| Session BI-TuE |
| Session: | 35 Years of NESAC/BIO II |
| Presenter: | Le Zhen, University of Washington, USA |
| Authors: | L. Zhen, University of Washington, USA M. Mecwan, University of Washington S. Zhang, University of Washington F. Simonovsky, University of Washington B.D. Ratner, University of Washington |
| Correspondent: | Click to Email |
Clotting is a major complication for blood contacting biomaterials intended to sustain normal blood flow (biomaterials used in vascular grafts, stents, artificial hearts, etc.). FDA-approved devices for use in the blood stream suffer from thrombotic complications and can be blood reactive even after years of implantation. Thus, highly blood-compatible biomaterials have been long-sought after but not yet achieved. Polyurethanes, with their readily tunable chemical and mechanical properties, represent one of the most widely used classes of biomaterials. We synthesized fluorinated polyurethane materials via a one-step, solvent-free, catalyst-free reaction. The ratio of CF3/CF2 can be tuned by varying the composition of the monomers. Electron spectroscopy for chemical analysis (ESCA) and attenuated total reflection Fourier-transform infrared spectroscopy (ATR-FTIR) were used to confirm the success of the reaction. 125I labeled albumin and fibrinogen are used in a competitive format to quantitatively study the adsorption of both proteins on the fluorinated polyurethanes. The retention of both proteins was quantified after elution with a sodium dodecyl sulfate (SDS) solution. Since fibrinogen is implicated in surface-induced clotting and albumin is a benign protein, we hypothesize that materials which tightly bind albumin and have reduced binding to fibrinogen will be less platelet activating and more blood compatible. Compared to non-fluorinated polyurethane and PTFE, the fluorinated polyurethane showed the highest albumin binding and retention. The albumin/fibrinogen ratio of the fluorinated polyurethane is higher than the non-fluorinated polyurethane and comparable to PTFE. These results show promise in blood-compatibility. We will further examine the albumin and fibrinogen adsorption to fluorinated polyurethanes with varying CF3/CF2 ratios and correlate composition to adsorption properties. The candidates optimized for high albumin binding and low fibrinogen binding from these experiments will be subjected to human platelet interaction studies to further test albumin hypotheses aimed at achieving enhanced blood-compatibility.