Pacific Rim Symposium on Surfaces, Coatings and Interfaces (PacSurf 2018)
    Biomaterial Surfaces & Interfaces Tuesday Sessions
       Session BI-TuE

Invited Paper BI-TuE3
The Evolution of Biomedical Surface Analysis at NESAC/BIO

Tuesday, December 4, 2018, 6:20 pm, Room Naupaka Salon 6-7

Session: 35 Years of NESAC/BIO II
Presenter: David Castner, University of Washington, USA
Correspondent: Click to Email
Biomedical surface analysis has undergone significant and numerous advances in the past decades in terms of improved instrumentation, introduction of new techniques, development of sophisticated data analysis methods, and the increasing complexity of samples analyzed. Comprehensive analysis of surfaces and surface immobilized biomolecules (peptides, proteins, DNA, etc.) with modern surface analysis instrumentation provides an unprecedented level of detail about the immobilization process and the structure of the immobilized biomolecules. Results from x-ray photoelectron spectroscopy (XPS or ESCA), time-of-flight secondary ion mass spectrometry (ToF-SIMS), near edge x-ray absorption fine structure (NEXAFS), surface plasmon resonance (SPR) and quartz-crystal microbalance with dissipation (QCM-D) biosensing, atomic force microscopy, and sum frequency generation (SFG) vibrational spectroscopy provide important information about the surface structure and composition of complex biomedical materials, as well as the attachment, orientation, conformation, etc. of biomolecules to those materials. However, even with the advances that have been achieved with these powerful surface analysis techniques, there still remain many significant challenges for biomedical surface analysis. These include characterizing the surface chemistry and structure of nanoparticles, determining the atomic level structure of proteins bound to surfaces, 3D imaging of cells and tissue sections, and maintaining biomolecules and materials in a biological relevant state when using ultra-high vacuum based analysis techniques. This talk will discuss the development of surface analysis tools at the National ESCA and Surface Analysis Center for Biomedical Problems (NESAC/BIO). Also discussed will be the role of well-defined standards to develop new biomedical surface analysis methods for characterizing more complex, biological relevant samples.