| Pacific Rim Symposium on Surfaces, Coatings and Interfaces (PacSurf 2016) | |
| Nanomaterials | Wednesday Sessions |
| Session NM-WeP |
| Session: | Nanomaterials Poster Session |
| Presenter: | Chiho Uemori, Nagoya University, Japan |
| Authors: | C. Uemori, Nagoya University, Japan W. Diono, Nagoya University T. Kon, Shalom Co. H. Kanda, Nagoya University M. Goto, Nagoya University, Japan |
| Correspondent: | Click to Email |
A liposome is a spherical vesicle having single or multiple lipid bilayers. Liposomes are generally composed of phospholipids. Lipophilic or hydrophilic compounds can be encapsulated in liposomes either in the phospholipid bilayer or in the inner aqueous phase. Liposomes are prepared conventionally by the Bangham method, the detergent depletion method, the reverse phase evaporation method, and emulsion method. Recently, supercritical carbon dioxide has been applied to produce liposome instead of organic solvent.
In this work, liposomes were produced utilizing supercritical fluid technology with ultrasonic mixing. Since no organic solvent was used in this process, solvent-free liposomes were prepared. As a lipid, we used sphingomyelin or phosphatidylcholine. Phospholipids and water were charged in high pressure vessel. Then, pressurized carbon dioxide was pumped into the vessel. The vessel was placed in thermostatted water bath and applied ultrasonic power to mix phospholipids, water, and carbon dioxide phase in order to produce liposome structure. The obtained liposomes were identified as multi-lamella form by TEM analysis. The effects of operation parameters such as ultrasonic frequency, pressure, and temperature, particle size of liposomes were studied.