Invited Paper BI-WeM4
Reconstruction Process and Orientation of Membrane Proteins in Artificial Cell Membrane Systems

Wednesday, December 14, 2016, 9:00 am, Room Milo

The lipid bilayer is the fundamental structure of cell membranes, at which the transportation of materials and signals in and out of cell membranes take place. Ion channels are one of representative membrane proteins promoting these reactions. They retain their proper structures and function only when they are incorporated in lipid bilayer membranes. It is necessary to reconstruct the membrane proteins into artificial lipid membranes to investigate the structure and function of membrane proteins out of cells. n this study we reconstructed the ion channels into solid-supported lipid bilayers (SLBs), which is an artificial lipid bilayer at solid-liquid interfaces, using proteoliposomes. We investigated the distribution and orientation of ion channels in the SLBs using fluorescence microscopy and atomic force microscopy (AFM).