| Pacific Rim Symposium on Surfaces, Coatings and Interfaces (PacSurf 2016) | |
| Biomaterial Surfaces & Interfaces | Tuesday Sessions |
| Session BI-TuP |
| Session: | Biomaterial Surfaces & Interfaces Poster Session |
| Presenter: | Cheng-Tien Hsieh, National Taiwan University, Taiwan, Republic of China |
| Authors: | G.H. Wu, National Taiwan University, Taiwan, Republic of China C.T. Hsieh, National Taiwan University, Taiwan, Republic of China S. Hsu, National Taiwan University,Taiwan, Republic of China |
| Correspondent: | Click to Email |
Waterborne biodegradable cationic polyurethane (WCPU) nanoparticle dispersion was synthesized by reaction of polycaprolactone, isophorone diisocyanate, and N-methyldiethanolamine under 75 °C and vigorous stirring under acidic condition. The nanoparticles dispersed in the aqueous medium were uniform with an average size of ~80 nm and a zeta potential of ~60 mV. The WCPU nanoparticle dispersion may be cast into films. The contact angle of the films was ~67°and the zeta potential was ~16 mV. The WCPU nanoparticles demonstrated excellent antibacterial activity against Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) (100% inhibition with a contact time of 3 h). Meanwhile, the antibacterial ratio of WCPU films to E. coli and S. aureus reached 100% after 24 h of contact. Moreover, The WCPU nanoparticles could be used as a transfection reagent without significant toxicity for concentrations less than 1000 mg/mL and showed the ability to condensate plasmid DNA. The transfection efficiency for HEK293T cells was ~60% after 48 h of transfection. Moreover, the WCPU dispersion can be made into microspheres conveniently. The WCPU dispersion prepared in the study has potential antibacterial and biomedical applications.