| Pacific Rim Symposium on Surfaces, Coatings and Interfaces (PacSurf 2016) | |
| Biomaterial Surfaces & Interfaces | Tuesday Sessions |
| Session BI-TuP |
| Session: | Biomaterial Surfaces & Interfaces Poster Session |
| Presenter: | Natalie Frese, Bielefeld University, Germany |
| Authors: | N. Frese, Bielefeld University, Germany D. Rhinow, Max Planck Institute of Biophysics, Germany A. Turchanin, Friedrich Schiller University, Germany N. Hampp, Philipps University, Germany A. Gölzhäuser, Bielefeld University, Germany |
| Correspondent: | Click to Email |
This presentation is about hybrid structures comprising carbon nanomembrane (CNM) as a functional substrate and oriented assembled purple membranes (PMs). CNMs are monomolecular cross-linked layers of aromatic amphiphilic molecules with lateral dimensions of several square centimeters and a thickness of about 1 nm. PM from Halobacterium salinarum is a membrane consisting of bacteriorhodopsin (BR), which is a light-driven proton pump, and lipids.
CNM has already been successfully tested as a substrate for electron cryo-microscopy of PM. To realize the oriented assembly of PM patches on CNM, we used a PM mutant, which has histidine (HIS) tags selectively on one side of the membrane and a nitroliotriacetic acid (NTA) terminated NBPT-CNM. The functionalized CNM has also been tested with different HIS-tagged proteins.