Pacific Rim Symposium on Surfaces, Coatings and Interfaces (PacSurf 2016)
    Biomaterial Surfaces & Interfaces Tuesday Sessions
       Session BI-TuP

Paper BI-TuP12
Cell based Tissue Engineering using Hydrogel Materials

Tuesday, December 13, 2016, 4:00 pm, Room Mauka

Session: Biomaterial Surfaces & Interfaces Poster Session
Presenter: Kangwon Lee, Seoul National University, Republic of Korea
Correspondent: Click to Email
Stem cell and growth-factor based therapies hold tremendous promise, but clinical effectiveness has been limited by transplanted cell death, efficacy of growth factors and limited control over cell fate in vivo. Macroporous biomaterials have been used in partially circumvent these problems by improving transplanted stem cell survival and controlling phenotype by providing molecular cues. In this presentation, we demonstrated strategies for bone repair. Mechanotransduction pathways have been harnessed to control stem cell behavior by manipulating the elasticity of both porous and non-porous materials. Here, we developed injectable, void-forming hydrogels in which critical biomaterial properties controlling stem cell behavior, including elasticity, could be decoupled from pore formation and cell confinement. Upon controlling of matrix elasticity, bone regeneration could be regulated and optimized. Next, CPC is promising for dental and craniofacial applications due to its ability to be injected or filled into complex-shaped bone defects and molded for esthetics, and its resorbability and replacement by new bone. The objective of this strategy was to investigate bone regeneration via novel macroporous CPC containing absorbable fibers, alginate hydrogel microbeads and growth factors in critical-sized cranial defects in rats. Macroporous CPC scaffolds containing porogen, fibers and microbeads with growth factors were investigated in rat cranial defects for the first time and had new bone up to 2-fold that of traditional CPC control at 4 weeks, and 3-fold that of traditional CPC at 24 weeks, and hence may be useful for dental, craniofacial