Pacific Rim Symposium on Surfaces, Coatings and Interfaces (PacSurf 2016)
    Biomaterial Surfaces & Interfaces Tuesday Sessions
       Session BI-TuP

Paper BI-TuP11
Histological Analysis of Bone Regeneration with Hydroxyapatite Isolated from Two Natural Sources (Gallus Domesticus and Sciaenops Ocellatus ) in Bone Defects induced in Tibiae of Rabbits

Tuesday, December 13, 2016, 4:00 pm, Room Mauka

Session: Biomaterial Surfaces & Interfaces Poster Session
Presenter: J.H. Luna-Domínguez, Universidad Autónoma de Tamaulipas, México
Authors: D.I. Balleza-Ovalle, Universidad Autónoma de Tamaulipas, México
H. Hernández-Cocoletzi, Benemérita Universidad Autónoma de Puebla, México
J.H. Luna-Domínguez, Universidad Autónoma de Tamaulipas, México
C.A. Luna-Lara, Universidad Autónoma de Tamaulipas, México
H. Tellez-Jimenez, Universidad Autónoma de Tamaulipas, México
E. Águila-Almanza, Benemérita Universidad Autónoma de Puebla, México
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In the field of dentistry, biomaterials that meet the requirements for optimal bone tissue formation have play a vital role for the treatment of bone reabsortion caused by periodontal disease, dental extractions or periapical lesions. Hydroxyapatite (HA) is a biocompatible nonabsorbable material chemically similar to the mineral component of bones and hard tissues, therefore, it can be used as a scaffold. HA can be isolated from natural sources by the thermal calcination method. The aim of this study is to assess the osteoconductive effect of hydroxyapatite isolated from two natural sources (Gallus domesticus and Sciaenops ocellatus ) by histological analysis applied in bone defects induced in tibiae of rabbits over a period of four weeks. In this experimental study, five healthy adult male rabbits of New Zealand strain, weighing approximately 3.5 kilograms, which were kept in cages according to the Mexican Official Standard NOM 062 Z00-1999 for the use of laboratory animals were used. Four defects with a size of 4 mm in diameter and 6 mm of deepness were made in the proximal metaphyseal planodiáfiso of both tibias and HA was applied to the defect according to the grouping. After four weeks of the surgery, both tibias were recovered in blocks containing all the graft area for histological analysis. Results: In the hydroxyapatite groups the new bone growth involved an area of 78.53% (Sciaenops ocellatus) and 72.23% (Gallus domesticus). The control group involved 15.97%. Conclusion: After four weeks, HA groups shown to be osteoinductive agents and they allowed the growth of bone tissue to a higher growth rate and bone of higher quality than in the control group.