AVS 64th International Symposium & Exhibition
    Tandem MS Focus Topic Monday Sessions
       Session TM-MoA

Invited Paper TM-MoA5
Coupling Front-end Electron Transfer Dissociation to Ultra-High Field FTICR-MS

Monday, October 30, 2017, 3:00 pm, Room 5 & 6

Session: Applications in Mass Spectrometry Imaging using Tandem MS
Presenter: Chad Weisbrod, The National High Magnetic Field Laboratory
Authors: C. Weisbrod, The National High Magnetic Field Laboratory
D.F. Smith, The National High Magnetic Field Laboratory
L.C. Anderson, The National High Magnetic Field Laboratory
L. He, The National High Magnetic Field Laboratory
A.G. Marshall, The National High Magnetic Field Laboratory
C.L. Hendrickson, The National High Magnetic Field Laboratory
Correspondent: Click to Email
Tandem MS is an indispensable tool of the mass spectrometrist. It enables structural elucidation and aides in unambiguous identification of precursor ions. Many means of performing tandem MS exist and can largely be categorized into three distinct groups: collision-, electron-, and photon-based. Each group has its own set of analytical merits and must be considered carefully when choosing which will best suit the analytical demand. Further, tandem MS can occur in-space or in-time which are unique to specific mass spectrometer configurations. A brief discussion of these categories of tandem MS will be given along with their relative strengths and weaknesses. A justification for our incorporation of front-end electron transfer dissociation (FETD) within the 21 T FTICR-MS at NHMFL will also be discussed. The 21T FT ICR-MS at NHMFL was constructed to achieve extraordinary performance with respect to top-down analysis. This is achieved by the increased field strength and the culmination of several technologies included during its construction. Here we focus on the inclusion of front-end electron transfer dissociation (FETD) coupled with an external multipole storage device (MSD), which allows for analysis of larger cumulative ion targets than ever before and lessens the need transient summing. We demonstrate linear operational range in terms of cumulative ion target (<5.0E4-3E7 total charges) and mass spectra with very high sequence coverage, in-spectrum dynamic range, and mass measurement accuracy despite the large cumulative injection targets. We show performance of FETD applied to standard proteins (3-30 kDa), human cell lysate samples, and monoclonal antibodies.