| AVS 64th International Symposium & Exhibition | |
| Plasma Processing for Biomedical Applications Focus Topic | Tuesday Sessions |
| Session PB+BI+PS-TuM |
| Session: | Plasma Medicine |
| Presenter: | Kenji Ishikawa, Nagoya University, Japan |
| Authors: | K. Ishikawa, Nagoya University, Japan R. Furuta, Nagoya University, Japan K. Takeda, Meijo University, Japan T. Ohta, Meijo University, Japan M. Ito, Meijo University, Japan H. Hashizume, Nagoya University, Japan H. Tanaka, Nagoya University, Japan H. Kondo, Nagoya University, Japan M. Sekine, Nagoya University, Japan M. Hori, Nagoya University, Japan |
| Correspondent: | Click to Email |
Non-equilibrium atmospheric-pressure plasma (NEAPP) affects cancer cells not only directly1 but also indirectly through exposure of cells to medium irradiated beforehand with NEAPP (i.e., plasma-activated medium [PAM]).2 Recent studies have revealed that NEAPP irradiation generates reactive oxygen and nitrogen species (RONS) in the gas phase and relatively long-lived RONS such as hydrogen peroxide, nitrites and nitrates in the aqueous phase.3 To further elucidate a cell-death mechanism in more detail, the present study focused on the direct analysis of PAM-induced intracellular molecules such as lipids, acylglycerol, triglyceride, adiposome in HeLa cells as cervical cancer cells. Lipid droplets (LDs) are dynamic organelles with complex and interesting biological functions that go beyond mere energy storage and are important in lipid homeostasis and metabolism. To evaluate LDs, coherent anti-Stokes Raman scattering (CARS) microscopy was used. The observation-results by multiplex coherent anti-Stokes Raman scattering (CARS) microscopy elucidated the mechanism underlying the apoptosis of HeLa cells in cultivating in PAM, leading to be simultaneously occurred the exhaustion of LDs in the cells in contrast to the accumulation, while the activation of caspase-3/7 was induced, though accumulation in lipid droplets (LDs) and lipid metabolism in the normal apoptosis of HeLa cells with activation of caspase-3/7 was previously reported.
Acknowledgement: This study was supported in part by the JSPS-KAKENHI (No. 24108002).
1 S. Iseki et al., Appl. Phys. Lett. 100, 113702 (2012); 2 H. Tanaka et al., Plasma Med. 2, 207 (2012); 3 N. Kurake et al., Arch. Biochem. Biophys. 605, 102 (2016).