| AVS 64th International Symposium & Exhibition | |
| Biomaterial Interfaces Division | Thursday Sessions |
| Session BI+AS+SA-ThM |
| Session: | Characterisation of Biological and Biomaterial Surfaces |
| Presenter: | Michael Taylor, University of Washington |
| Authors: | M.J. Taylor, University of Washington M.J. Hawker, Colorado State University M.N. Mann, Colorado State University G.E. Hammer, University of Washington E.R. Fisher, Colorado State University D.J. Graham, University of Washington L.J. Gamble, University of Washington |
| Correspondent: | Click to Email |
Biopolymers show increasing usage in medical device technologies including joint replacement, stents and tissue engineered supports. (polymer scaffolds). Barriers to successful use of biopolymer usage for medical devices can include ineffective interaction of biological systems with the biopolymer and biofilm formation. Historically, developing medical devices with antibacterial properties have involved inclusion of silver or copper dopants as they facilitate bacterial membrane rupture. Bacterio-static coatings provide an alternative approach by generating a hydrophobic surface that prevents colonisation by reversible adhesion via van der Waals forces prior to anchoring strongly with adhesion structures such as pili. Plasma enhanced chemical vapor deposition (PECVD) is a cheap yet powerful method of introducing chemical functionalities to surfaces as the low temperature high energy process may be used to couple a variety of monomers to biomaterial surfaces. Previous evidence provided by Fisher and coworkers showed that PECVD may be utilised to produce antifouling coatings by modifying polycaprolactone (PCL) with fluorinated organic compounds1, however the porous morphology of scaffolds required for vascularisation also provides multiple points of attachment for the critical first step in biofilm formation. It is therefore necessary determine the effectiveness of PECVD throughout the scaffold. For this we employ time-of-flight secondary ion mass spectrometry (ToF-SIMS) and X-ray photoelectron spectroscopy (XPS) to analyse the surface modification of porous polymer scaffolds.
ToF-SIMS imaging coupled with X-ray photoelectron spectroscopy (XPS) provides a powerful combination of high resolution imaging and elemental quantitative analysis that allows a detailed analysis of the surface. Herein we apply this combination of analysis methods for the determination and quantification of fluorocarbon distribution across a PCL scaffold modified with octafluoropropane by PECVD, determining that a treatment time of 20 minutes Introduces a homogeneous distribution of fluorocarbon film throughout the construct cross section whereas lower treatment times produces a gradient distribution of fluorocarbon, as measured via CF+ and CF3+ signals
(1) Hawker, M. J.; Pegalajar-jurado, A.; Fisher, E. R. Conformal Encapsulation of Three-Dimensional, Bioresorbable Polymeric Sca Ff Olds Using Plasma-Enhanced Chemical Vapor Deposition. 2014.