| AVS 64th International Symposium & Exhibition | |
| Biomaterial Interfaces Division | Tuesday Sessions |
| Session BI+AS+MI+SA-TuA |
| Session: | Bio from 2D to 3D: Challenges in Fabrication and Characterization & Flash Presentations |
| Presenter: | Axel Rosenhahn, Ruhr-University Bochum, Germany |
| Authors: | A. Rosenhahn, Ruhr-University Bochum, Germany S. Stuhr, Ruhr-University Bochum, Germany C. Rumancev, Ruhr-University Bochum, Germany T. Senkbeil, Ruhr-University Bochum, Germany T. Gorniak, Ruhr-University Bochum, Germany A. von Gundlach, Ruhr-University Bochum, Germany J. Reinhardt, Ruhr-University Bochum, Germany Y. Yang, ESRF, France P. Cloetens, ESRF, France M. Grunze, Karlsruhe Institute of Technology (KIT), Germany J. Garrevoet, DESY, Germany G. Falkenberg, DESY, Germany W. Schröder, DESY, Germany |
| Correspondent: | Click to Email |
Nanoprobe X-ray fluorescence (nano-XRF) analysis allows spatially resolved imaging with chemical sensitivity. Approaching the diffraction limit at the next generation of storage rings, both, spatial resolution and brilliance are going to be strongly enhanced for nano-XRF experiments. For biological samples, the combination of nano-XRF with cryogenic sample environments allows to understand elemental distributions in cells with minimum preparation artefacts. In addition, the cryo-protected samples provide enhanced resistance against radiation damage, which is particularly important for the high photon densities at modern synchrotron sources. Three different applications of cryo-nano-XRF will be presented. For single melanosomes, the technique enabled us to prove the core-shell organization of the organelles using metals as surrogate markers. As second application, the distribution of metals in single, adherent cells was directly imaged without the requirement of additional markers. Finally, marine adhesives of diatoms were analyzed and the occurrence of metals are linked with the known organic constituents in the EPS of diatoms. In all three cases, the detection of metal distribution has provided a new view on the investigated samples. The cryogenic sample environments proved to be the key to apply synchrotron radiation to all three types of biological samples. The data will also be discussed in relation to the perspectives of new implementations that will enable fast cryo-3D imaging in the future.