AVS 62nd International Symposium & Exhibition
    Thin Film Wednesday Sessions
       Session TF+AS+BI-WeA

Paper TF+AS+BI-WeA8
SAM-based Models of Cell Surfaces to Study the Interactions with Lectins and Bacterial Fimbriae

Wednesday, October 21, 2015, 4:40 pm, Room 114

Session: Thin Films for Biological and Biomedical Applications
Presenter: Andreas Terfort, University of Frankfurt, Germany
Authors: A. Terfort, University of Frankfurt, Germany
K. Lindhorst, University of Kiel, Germany
Correspondent: Click to Email
Biologically important events such as cell-cell adhesion or infection typically start by directed and selective interactions with the highly glycosylated layer surrounding most eukaryotic cells. This layer, called the glycocalyx, consists of intricate glycopolymers, which – although in apparent disorder – clearly identify the cells. It is therefore of paramount interest to understand, which structural elements are important for the cell identification.

Self-assembled monolayers (SAM) can be used to simulate the chemical and sterical environment within such a glycocalyx. For this, glycosides are attached to oligoethyleneglycol (OEG) chains, which simulate the hydrogel matrix for the respective receptor. In this talk, we will focus on mannose-derivatives, which can be selectively recognized either by a lectin, concanvalin A, or by the adhesive fimbriae (tiny protein extrusions) of E. coli cells.

We would like to present different strategies for the construction of such SAMs [1,2] and discuss the advantages and disadvantages of these approaches. In extension of the mostly static systems, we will also present an approach to dynamically reorient the glycoside at the interface to determine the influence of steric factors on surface recognition [3].

References

[1] Kleinert, M.; Winkler, T.; Terfort, A.; Lindhorst, T.K. Org. Biomol. Chem.6, 2118-2132 (2008)

[2] Grabosch, C.; Kind, M.; Gies, Y.; Schweighöfer, F.; Terfort, A.; Lindhorst, T. K. Org. Biomol. Chem.11, 4006-4015 (2013).

[3] Weber, T.; Chandrasekaran, V.; Stamer, I.; Thygesen, M.B.; Terfort, A.; Lindhorst, T.K. Angew. Chemie Int. Ed.53, 14583–14586 (2014).