| AVS 62nd International Symposium & Exhibition | |
| Surface Modification of Materials by Plasmas for Medical Purposes Focus Topic | Thursday Sessions |
| Session SM+AS+BI+PS-ThM |
| Session: | Plasma Processing of Biomaterials |
| Presenter: | Kensaku Goto, Osaka University, Japan |
| Authors: | K. Goto, Osaka University, Japan D. Itsuki, Osaka University, Japan M. Isobe, Osaka University, Japan S. Sugimoto, Osaka University, Japan S. Miyamoto, Osaka University, Japan A. Myoui, Osaka University, Japan H. Yoshikwa, Osaka University, Japan S. Hamaguchi, Osaka University, Japan |
| Correspondent: | Click to Email |
Polystyrene is a widely used cell-culture plate material. Currently cell culture plates on the market include those whose inner surfaces are covered with amino and/or carbonyl groups for a better control of cell adhesion to the plate surfaces. Such functional groups on a cell culture plate surface may immobilize glycoproteins or other biopolymers that function as extracellular matrices (ECM) and thus affect the environments where the cells are cultured. The goal of this research is to understand how such functional groups, especially amino groups, are formed on a polystyrene surface, depending on the deposition methods. Of particular interest are plasma-based methods of surface functionalization. In this study, we have observed experimentally how exposure of N2/H2 or N2/CH3OH plasmas to polystyrene surfaces form amino-group-like structures and also examined using molecular dynamics (MD) simulation how a polystyrene surface interacts with incident energetic ions such as NH3+ as well as abundant low-energy radicals such as NH2 under conditions similar to our experiments. In the experiments, we used parallel-plate discharges with an inverter power supply whose peak-to-peak voltage was about 3kV and frequency was 20kHz at a relatively high gas pressure of 250 - 2,500 Pa. In MD simulation, we used a simulation code with interatomic potential functions that had been developed in-house based on quantum mechanical calculations of atomic interactions involved in this system. Results of MD simulations under the conditions similar to plasma enhanced chemical vapor deposition (PE-CVD) by ammonia plasmas or cyclopropylamine (CPA) [1] suggest that, with energetic ion bombardment, amino groups tend to be broken to form new covalent bonds by ion bombardment. Preliminary results of cell culture experiments with plasma-treated polystyrene cell plates will be also reported.
[1] A. Manakhov, L. Zajickova, et al. Plasma Process. Polym.11, (2014) 532.