| AVS 62nd International Symposium & Exhibition | |
| Surface Modification of Materials by Plasmas for Medical Purposes Focus Topic | Thursday Sessions |
| Session SM+AS+BI+PS-ThA |
| Session: | Plasma Processing of Biomaterials and Biological Systems |
| Presenter: | Kenji Ishikawa, Nagoya University, Japan |
| Authors: | K. Ishikawa, Nagoya University, Japan R. Furuta, Nagoya University, Japan K. Takeda, Nagoya University, Japan T. Nomura, Meijo University, Japan T. Ohta, Meijo University, Japan H. Hashizume, Nagoya University, Japan H. Kondo, Nagoya University, Japan M. Ito, Meijo University, Japan M. Sekine, Nagoya University, Japan M. Hori, Nagoya University, Japan |
| Correspondent: | Click to Email |
Applications of nonequilibrium atmospheric pressure plasma (NEAPP) to the medical field have been reported in recent years. However, a mechanism of interactions between NEAPP and living cells has not been yet elucidated comprehensively. Our strategy for elucidation of plasma-biomaterial interactions is to observe reactions in situ at real time. By applying nonlinear optical spectroscopic techniques, the vibrational sum-frequency-generation (SFG) and multiplex coherent anti-Stokes Raman scattering (CARS) microscopy, which are a beneficial tool for addressing best sensitivity at surface and interface, have been used in this study. By using SFG, we have explored topmost surface modification after the interaction between plasma and biopolymeric materials. For the NEAPP-induced reactions on budding yeasts as an eukaryotic cell model, a two-dimensional mapping of budding yeasts treated by the plasma using the CARS microscopy was observed with fluorescence label-free contrasts of chemical vibrational nature. The biomedical imaging of cell membranes, intracellular organelles, nucleus and so forth, was revealed to decompose intracellular membrane by exposure of plasma-generated chemically reactive species, especially for induction of lipid peroxidation. These results will be useful for understanding the plasma induced reactions in the plasma medicine.