Paper SA-MoM6
Nanoscale Tomography and Spectroscopy with the HZB X-ray Microscope
Monday, October 19, 2015, 10:00 am, Room 112
The Helmholtz-Zentrum Berlin (HZB) operates a full-field transmission X-ray microscope (TXM) at the BESSY II electron storage ring. The advanced optical setup of the HZB-TXM permits NEXAFS spectroscopic applications as well as correlative fluorescence and nanoscale tomographic imaging of cryogenic fixed cells in a fully hydrated state. An overview of recent results in material and life sciences will be presented [1-8]. Investigations on TiO2 nanostructures using NEXAFS spectromicroscopy at the Ti-L- and O-K-absorption edges will be shown [1,2]. Additionally, first results on the nanoscale morphology of organic solar cells will be discussed. Reconstructions taken from 3D X-ray datasets allow to visualize sub-cellular ultrastructures in mammalian and plant cells e.g. algae. Quantitative studies as e.g. the number of cell organelles in the cell volume are possible [4]. Scientific findings on the nucleation of hemozoin in Plasmodium falciparum by nanoscale tomography with the HZB-TXM will be demonstrated [5]. Studies of the interaction of viruses like the Herpes virus [6] or vaccinia virus [7] with mamillian cells will be presented. In addition, nanoscale X-ray tomography paves the way to a better understanding of the interaction of nanoparticles with cells [8].
[1] P. Guttmann et al., Nature Photonics 6 (2012), 25-29
[2] K. Henzler et al., Nano Letters 13 (2013), 824-828
[3] G. Schneider et al., Nature Methods 7 (2010), 985-987
[4] E. Hummel et al., PLoS ONE 7 (12) (2012), e53293
[5] S. Kapishnikov et al., PNAS (2012) DOI: 10.1073/pnas.1118120109
[6] C. Hagen et al., J. Struct. Biol. 177 (2012), 193-201
[7] FCO J. Chichon et al., J. Struct. Biol. 177 (2012), 202-211
[8] D. Drescher et al., Nanoscale 5 (2013), 9193-9198