| AVS 62nd International Symposium & Exhibition | |
| Biomaterial Interfaces | Tuesday Sessions |
| Session BI-TuA |
| Session: | Cells and Microorganisms at Surfaces |
| Presenter: | Axel Rosenhahn, Ruhr-University Bochum, Germany |
| Authors: | S. Maleschlijski, Karlsruhe Institute of Technology (KIT), Germany G.H. Sendra, Karlsruhe Institute of Technology (KIT), Germany S. Bauer, Karlsruhe Institute of Technology (KIT), Germany A. Di Fino, Newcastle University, UK L. Leal-Taixe, Leibnitz University Hannover, Germany T. Ederth, Linköping University, Sweden N. Aldred, Newcastle University, UK B. Liedberg, NTU Singapore A.S. Clare, Newcastle University, UK B. Rosenhahn, Leibnitz University Hannover, Germany A. Rosenhahn, Ruhr-University Bochum, Germany |
| Correspondent: | Click to Email |
The critical step in surface colonization by marine biofouling organisms is surface exploration and settlement of the sessile stages (larvae, spores). Barnacles are one of the most important biofouling organism and surface selection of their larvae is a highly selective process [1]. 3D stereoscopic tracking enables quantitative analysis of the pre-settlement behavior and thus to understand how larvae respond to chemical and physical surface cues. We developed a transportable, submersible stereoscopic system which can be applied to record three dimensional video data and to extract swimming trajectories of multiple, label-free objects. The pre-settlement ritual can be classified in different motion patterns which vary in characteristic parameters, such as distance to the surface, velocity, or the curvature of the motion [2]. In general the larvae favor both, liquid-solid and liquid-air interfaces. The distribution within the water column and the fraction of larvae exploring the solid surface is determined by its chemistry. Using different self assembled monolayers we found a positive correlation of the settlement probability with both, the fraction of larvae exploring the interface and their mean swimming velocity [3]. Thus, 3D tracking provides a predictor for settlement probability. A combination of stereoscopic tracking with imaging surface plasmon resonance reveals that a temporary adhesive is an important ingredient in the mechanosensing process. Surfaces with high settlement probability and low swimming speeds tend to bind the adhesive stronger than the sensory setae while inert surfaces with low settlement probability and high swimming speeds interact only very weakly.
[1] N. Aldred, A.S. Clare, Biofouling 2008, 24, 351–363.
[2] S. Maleschlijski, S. Bauer, A. DiFino, H. Sendra, A.S. Clare, A. Rosenhahn, J. Roy. Soc. Interf. 2014, 12(102), 20141104
[3] S. Maleschlijski, S. Bauer, A. Di Fino, G.H. Sendra, A.S. Clare, A. Rosenhahn, Biofouling 2014, 30(9), 1055