| AVS 62nd International Symposium & Exhibition | |
| Biomaterial Interfaces | Monday Sessions |
| Session BI+AS-MoM |
| Session: | Characterization of Biological and Biomaterials Surfaces (1) |
| Presenter: | Giacomo Ceccone, European Commission, Joint Research Centre, IHCP, Italy |
| Authors: | R. La Spina, European Commission, Joint Research Centre, IHCP, Italy V. Spampinato, European Commission, Joint Research Centre, IHCP, Italy I. Ojea, European Commission, Joint Research Centre, IHCP, Italy F.J. Rossi, European Commission, Joint Research Centre, IHCP, Italy D. Gilliland, European Commission, Joint Research Centre, IHCP, Italy GC. Ceccone, European Commission, Joint Research Centre, IHCP, Italy |
| Correspondent: | Click to Email |
It is recognized that detailed physico-chemical characterization of nanomaterials is becoming increasingly important both from the technological and from health and safety point of view. Moreover, an incomplete characterisation may inhibit or delay the scientific and technological impact of nanoscience and nanotechnology. However, nanomaterials characterization based on individual instrumental methods is a very challenging issue because their stability, coating and environmental effects may lead to outputs that are not very easy to interpret unequivocally. For this reason multiple analysis methods are needed to understand the nature of nanomaterials, especially if we consider that surface and interfaces are critical to the behaviours of nano-sized materials [1].
Surface chemical analysis methods, such as X-ray Photoelectron Spectroscopy (XPS) and Time of Flight Secondary Ion Mass Spectrometry, can provide an important contribution to more fully characterizing nanomaterials, so these methods should be more generally applied as part of a characterisation set of tools for nanomaterials and nanoparticles synthetized for different applications [2].
In this work, we have investigated the surface chemistry of nanoparticles, gold (AuNPS) and silica (SiO2NPs), functionalized with different thiols. In particular, the effects of sample cleaning by centrifugation and dialysis have been studied. Moreover, the challenges and problems related to sample preparation for the surface analysis will be also addressed and discussed The different steps of sample cleaning have been characterised by DLS, CPS and SEM, whilst the surface chemistry has been mainly assessed by XPS. Our results indicate that the cleaning process may influence the functionalization process. For instance, the AuNPs functionalized with CF3 terminated SAMs shows differences in the efficiency depending upon sample cleaning.
Finally, preliminary results about the behaviour of AuNPs-CF3 in protein solution (HSA) will be also presented.
[1] Baer D, et al., Anal. Bioanal. Chem., 2010, 396(3), 983–1002
[2] Grainger D and Castner D, Adv. Mater., 2008, 20, 867–877