AVS 61st International Symposium & Exhibition
    Surface Modification of Materials by Plasmas for Medical Purposes Focus Topic Thursday Sessions
       Session SM+AS+BI+PS-ThM

Paper SM+AS+BI+PS-ThM10
Plasma Treated Substrates Reduce Protein Adsorption

Thursday, November 13, 2014, 11:00 am, Room 315

Session: Plasma Processing of Antimicrobial Materials and Devices
Presenter: Marvin Mecwan, University of Washington
Authors: M. Mecwan, University of Washington
J. Stein, University of Washington
W. Ciridon, University of Washington
X. Dong, Eli Lilly and Company
B. Ratner, University of Washington
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Proteins irreversibly adsorb onto surface, causing losses from solution, denaturation, as well as aggregation. Hence, there have been recent efforts in the pharmaceutical industry to addressing the manufacture, packaging and delivery of protein-based pharmaceuticals. We propose the use of radio-frequency (RF) plasma deposition to create coatings on substrates relevant to the pharmaceutical industry—glass, stainless steel and cyclic olefin polymer (COP). The monomers of choice were acrylic acid (AA) and tetraglyme (TG) (hydrophilic), and perfluoropropylene (C3F6) and perfluoromethyl vinyl ether (C3F6O) (hydrophobic). All monomers were successfully plasma coated on all substrates, and did not delaminate as was determined from survey and detailed ESCA scans. Furthermore, no peaks associated with the substrates were seen in the scans, which indicate that the plasma coating are at least 100Å thick. Protein adsorption studies were carried out using 0.1mg/mL solution of I-125 tagged bovine IgG by adsorbing the tagged protein on the plasma treated substrates for an hour. All hydrophilic monomer plasma treated substrates had lesser protein adsorbed on their surfaces (< 2ng/cm2) as compared to hydrophobic plasma treated substrates (10-14 ng/cm2). This is in comparison to untreated controls that had 200-300 ng/cm2 protein adsorbed on the surface. Furthermore, following ISO 10993-5 guidelines, by performing cytotoxic studies using NIH-3T3 fibroblasts all plasma treated substrates were determined to be non-cytotoxic. Hence, these results indicate that radio-frequency plasma treatment could lead to a new generation of surfaces that will be particularly effective for protein manufacture, storage and delivery. Future studies will be aimed at determining plasma coating thickness, protein aggregation assessment as well as studying the bonding strength of the proteins to the plasma treated surfaces.