AVS 61st International Symposium & Exhibition
    Helium Ion Microscopy Focus Topic Thursday Sessions
       Session HI+2D+AS+BI+MC-ThM

Paper HI+2D+AS+BI+MC-ThM12
Helium Ion Microscopy of Biological Cells

Thursday, November 13, 2014, 11:40 am, Room 316

Session: Fundamental Aspects and Imaging with the Ion Microscope
Presenter: Natalie Frese, University of Bielefeld, Germany
Authors: N. Frese, University of Bielefeld, Germany
A. Beyer, University of Bielefeld, Germany
M. Schürmann, University of Bielefeld, Germany
B. Kaltschmidt, University of Bielefeld, Germany
C. Kaltschmidt, University of Bielefeld, Germany
A. Gölzhäuser, University of Bielefeld, Germany
Correspondent: Click to Email

In this presentation HIM images of biological cells are presented. The presented study focuses on neuronal differentiated human inferior turbinate stem cells, mouse neurons and mouse fibroblasts. The cells were prepared by critical point drying or freeze drying and a flood gun was used to compensate charging, so no conductive coating was necessary.

Therewith, extremely small features at native cell surfaces were imaged with an estimated edge resolution of 1.5 nm. Due to the size of the structures and the preparation methods of the cells the observed features could be an indicator for lipid rafts. This hypothesis will be discussed.