AVS 61st International Symposium & Exhibition
    Biomaterial Interfaces Tuesday Sessions
       Session BI+AS-TuA

Paper BI+AS-TuA9
Analysis of Peptide Microarrays on Si Using ToF-SIMS

Tuesday, November 11, 2014, 5:00 pm, Room 317

Session: Characterization of Biointerfaces
Presenter: James A. (Tony) Ohlhausen, Sandia National Laboratories
Authors: J.A. Ohlhausen, Sandia National Laboratories
C. James, Sandia National Laboratories
D. Smith, HealthTell
S.A. Johnston, Arizona State University
N. Woodbury, Arizona State University
Correspondent: Click to Email
A microarray containing over 1200 each 200µm diameter spots consisting of various length peptide chain monolayers was analysed using Time-of-flight Secondary Ion Mass Spectrometry (ToF-SIMS). This peptide microarray was created using lithographic processes where chains of peptides were built one amino acid at a time. A silane coupling agent was used to attach the peptides to the oxide surface creating a monolayer of peptides directly bonded to the Silicon oxide surface. By tracking ion fragments corresponding to specific amino acids, usually immonium ions, we show that contrast consistent with the number of individual amino acid units in a given peptide dot is generally seen. While some immonium ions are not specific enough to generate clear contrast patterns, most can be used to verify the presence expected amino acids in each peptide dot. Additionally, some amino acids were not found to generate a specific fragment for identification in the positive secondary ion mode.

**Sandia National Laboratories is a multi-program laboratory managed and operated by Sandia Corporation, a wholly owned subsidiary of Lockheed Martin Corporation, for the U.S. Department of Energy’s National Nuclear Security Administration under contract DE-AC04-94AL85000.