AVS 61st International Symposium & Exhibition
    Biomaterial Interfaces Monday Sessions
       Session BI+AS-MoM

Invited Paper BI+AS-MoM5
High Throughput BioMaterials Screening using Microarrays and High Information Content Imaging Methods

Monday, November 10, 2014, 9:40 am, Room 317

Session: Biomolecules & Biomaterials Interfaces
Presenter: John Brennan, McMaster University, Canada
Authors: S. Boudjabi, McMaster University, Canada
D. Covelli, McMaster University, Canada
M. Keramane, McMaster University, Canada
E. Luckham, McMaster University, Canada
J.D. Brennan, McMaster University, Canada
Correspondent: Click to Email
This presentation will highlight recent work in the area of high throughput screening of biologically modified surfaces for production of biosensors, protein and cell microarrays, and non-fouling surfaces. Using robotic material synthesis and assay systems and a combination of contact and noncontact microarray printing, we have produced several libraries of biomaterials with a wide range of chemical compositions based on acrylate, silicone and silica-based polymers. Using silica-based materials as an example, the presentation will show the workflow utilized to develop new bioactive polymer materials for generation of bioactive and stealth materials and coatings. This includes methods to produce several thousand materials very rapidly via printing, rapid imaging tools and assays for screening to identify "hits" that show a desired property (i.e., high bioactivity, low non-specific binding), and methods for detailed material analysis using a range of imaging methods based on fluorescence, XPS, MALDI-MS/MS, FTIR and SPR to fully characterize the properties of biologically active materials. Methods for mining and analyzing the large datasets produced using our in-house developed Biointerfaces Research Gateway will be described.