AVS 60th International Symposium and Exhibition | |
Nanometer-scale Science and Technology | Tuesday Sessions |
Session NS+AS+EN+SS-TuA |
Session: | Nanoscale Catalysis and Surface Chemistry |
Presenter: | T. Shaw, Clark Atlanta University |
Authors: | T. Shaw, Clark Atlanta University M.D. Williams, Clark Atlanta Unversity J. Reed, Clark Atlanta University |
Correspondent: | Click to Email |
Biotin-avidin technology is a widely explored interaction in bioscience. Biotin’s affinity for the protein avidin, makes it ideal for protein and nucleic acid detection or purification methods. This strong interaction if often used in pretargeting strategies for cancer treatment. In most cases a probe molecule (antibody) is connected to a marker molecule (fluorophore or nanoparticle) through the biotin-avidin bridge. Biotinylated nanoparticles can play a role in improving this interaction and creating an electronic or optical detection method. Polyaniline is a polymer which can be easily functionalized to be specific for various biomolecules and has ideal sensor characteristics. In this study we will design a process to functionalize polyaniline with biotin to create a biotin-avidin biosensor. We began with a 2-aminophenol which is a hydroxyl substituted aniline monomer. This monomer undergoes polymerization to yield 2-hydroxy polyaniline. The polymer’s hydroxyl group was functionalized by Steglich esterification which refluxes a carboxylic acid with an alcohol. This esterification drives the reaction and dehydrates the products shifting the equilibrium towards the product. In this reaction DCC (dicyclohexylcarbodiimide) activates the carboxylic acid of biotin to further reaction and DMAP (4-dimethlyaminopyridine) acts as the acyl transfer catalyst. The biotinylated polyaniline derivative was characterized using FT-IR spectroscopy, 1H NMR spectroscopy, UV-VIS spectroscopy, and Scanning Electron Microscopy.