| AVS 60th International Symposium and Exhibition | |
| Biomaterial Interfaces | Wednesday Sessions |
| Session BI-WeM |
| Session: | Cell-Surface Interactions |
| Presenter: | D.E. Barlow, U.S. Naval Research Laboratory |
| Authors: | D.E. Barlow, U.S. Naval Research Laboratory P.A. Fulmer, U.S. Naval Research Laboratory T.J. O'Shaughnessy, U.S. Naval Research Laboratory K.P. Fears, U.S. Naval Research Laboratory J. Morabito, U.S. Naval Research Laboratory R. Stine, U.S. Naval Research Laboratory S.P. Mulvaney, U.S. Naval Research Laboratory B.R. Ringeisen, U.S. Naval Research Laboratory |
| Correspondent: | Click to Email |
Vibrational spectroscopies are valuable methods for non-destructive characterization of biochemical functionality and physiological changes in stem cells. To date, the primary approaches have almost exclusively used either Raman microscopy or Fourier transform infrared (FTIR) microscopy in transmission or reflection configurations. Another FTIR approach is the interfacially sensitive attenuated total reflectance (ATR) configuration which has often been used for in situ characterization of buried cell-substrate interfaces with live microorganisms such as bacteria. However, so far, the method has rarely been used with mammalian cells. Our approach provides a useful complimentary method for which multiple live specimens can be kept under controlled environmental conditions and analyzed by ATR-FTIR over multi-week periods. As a first step in employing in situ ATR-FTIR, we will present results demonstrating early detection of osteogenic differentiation of live human mesenchymal stem cells (hMSC’s). In comparison to control hMSC spectra, hydroxyapetite related bands are clearly observed for cells in osteogenic media within 24 hours. Further hydroxyapetite formation and differentiation characteristics were observed over 2 week periods. Additional results will also be presented comparing interfacial spectra of live hMSC’s on surfaces with varying properties, including poly-D-lysine/laminin coated substrates, graphene, and nanocrystaline hydroxyapetite.