| AVS 60th International Symposium and Exhibition | |
| Biomaterial Interfaces | Tuesday Sessions |
| Session BI-TuP |
| Session: | Biomaterials Interfaces Poster Session |
| Presenter: | P. Bao, University of Leeds, UK |
| Authors: | P. Bao, University of Leeds, UK G.R. Heath, University of Leeds, UK J. Roth, University of Leeds, UK B. Johnson, University of Leeds, UK M. Cheetham, University of Leeds, UK R.J. Bushby, University of Leeds, UK S.D. Evans, University of Leeds, UK |
| Correspondent: | Click to Email |
Supported lipid bilayers have found widespread use a model system for the investigation of basic properties of cell membranes, as well as for the development of diagnostic assays and in biosensing [1-3]. The potential of supported lipid bilayers however is still not fully realised considering the possibility of fine tuning the surface charge, fluidity, and organisation at the molecular level. In this work we have been interested in using the dynamic nature of the planar membrane as a substrate to support the crystallisation of monolayers gold nanoparticles. Two different methods of crystal formation have been investigated. In the first negatively charged gold nanoparticles were attached to a neutral lipid bilayer via cholesterol anchors and concentrated via the application of an electric field within the plane of the membrane [4-8]. This resulted regions of high nanoparticle density. We describe results of electric field annealing and the role of nanoparticle concentration on the structures formed. In the second approach we first created a bilayer displaying a phase-separation, into liquid ordered and discorded regimes and containing a small fraction of positively charged lipid. Interestingly the gold nanoparticles spontaneously assembled on the liquid ordered regimes to form quasi-crystals of nanoparticles.
The presentation will describe our combined fluorescence microscope and atom force microscope (AFM) studies on these systems and the role of temperature on assembly formation as well as the mechanism related to the crystallisation. Our results may inspire a wider application lipid bilayers as dynamic structures for the directed assembly of inorganic materials.
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