| AVS 60th International Symposium and Exhibition | |
| Biomaterial Interfaces | Monday Sessions |
| Session BI+AS+IS+NL-MoM |
| Session: | Surfaces to Control Cell Response |
| Presenter: | A. Rosenhahn, Ruhr-University Bochum, Germany |
| Authors: | A. Rosenhahn, Ruhr-University Bochum, Germany M. Hanke, Karlsruhe Institute of Technology, Germany C. Christophis, Karlsruhe Institute of Technology, Germany I. Taubert, University of Heidelberg, Germany N. Baran, University of Heidelberg, Germany P. Wuchter, University of Heidelberg, Germany A.D. Ho, University of Heidelberg, Germany |
| Correspondent: | Click to Email |
Especially for leukemic and haematopoietic cells, the interaction with their microenvironment is of upmost importance for extravasation and homing. One key mechanism is the interaction of the CD44 receptor with extracellular hyaluronan (HA) binding motifs. To quantitatively assess the interaction, a microfluidic experiment has been developed that allows studying the interaction of cells with interfaces under well-defined flow conditions [1]. Shear flow activated catch bond interaction is well characterized for selectin mediated extravasation of leukocytes [2]. We recently found that also the CD44 interaction with HA requires a minimum shear stress to become activated and enable cells to roll on HA surfaces [3]. Similar critical shear values were found for rolling on mesenchymal stroma cells, which are present in the bone marrow niche creating the microenvironment required for haematopoietic stem cell renewal. Interestingly not only hematopoietic stem cells but also acute leukemic blasts show a shear flow induced rolling. The proportion of rolling cells will be discussed on the basis of the pathogenesis of the disease.
[1] C. Christophis, M. Grunze, A. Rosenhahn, PCCP 2010, 12, 4498.
[2] E.B. Finger, K.D. Puri, R. Alon, M.B. Lawrence, U.H. von Andrian, T.A. Springer, Nature 1996, 379, 266
[3] C. Christophis, I. Taubert, G. Meseck, M. Schubert, M. Grunze, A. D. Ho, A. Rosenhahn, Biophys. J. 2011, 101, 585.