| AVS 60th International Symposium and Exhibition | |
| Biomaterial Interfaces | Monday Sessions |
| Session BI+AS+IS+NL-MoM |
| Session: | Surfaces to Control Cell Response |
| Presenter: | P.Y. Wang, Swinburne University of Technology, Australia |
| Authors: | P.Y. Wang, Swinburne University of Technology, Australia P. Kingshott, Swinburne University of Technology, Australia |
| Correspondent: | Click to Email |
The control of cell behaviour on surfaces is the key to a broad range of biomedical applications. Biomaterial surfaces with tuneable surface topographies and chemistries can profoundly influence the development of advanced biomaterials used in applications including tissue engineering and regenerative medicine. Recently, we developed an elaborate and feasible method to display an ordered surface topography with tuneable surface chemistry using binary colloidal crystal particles. Using this binary colloidal system, various combinations of particle size and surface chemistry can be readily employed. In this study, two combinations of binary colloidal crystals, i.e. PS-COOH (2 µm)/PMMA (0.4 µm) and SiO2 (2 µm)/PMMA (0.4 µm) were assembled on ozone-treated silicon wafers. The preliminary results of cell attachment and morphology of L929 fibroblasts and MG63 osteoblasts were studied after 24h.
In general, cells had a small projection area rather than fully spread morphology on the crystal surfaces compared with the flat control. Fibroblasts have abundance of cell protrusions called filopodia which can be observed using scanning electron microscopy (SEM), whilst osteoblasts don’t have. Fibroblasts had long and thin extended filopodia on the PS/PMMA crystal surfaces, whilst they had short and thick filopodia on the SiO2/PMMA crystal surfaces. Regarding the surface chemistry, both SiO2 and PMMA particles were not as favourable as the PS-COOH particles for fibroblasts attachment, and resulted in the cell projection area on the PS/PMMA being larger compared to the SiO2/PMMA crystal surfaces. On the contrary, the cell projection area of osteoblasts didn’t have significant differences between these two crystal surfaces. After fibronectin coating, cell projection area of osteoblasts on SiO2/PMMA crystal surfaces increased significantly, whilst fibroblasts didn’t, suggesting that different cell types respond to surfaces differently.
These results show for the first time that cell-substrate interactions can be easily controlled by precise positioning of different particles with various sizes and chemistries. The present results will help gain a more thorough understanding of cell-material interactions benefiting the development of advanced biomaterials and materials for tissue engineering.