| AVS 60th International Symposium and Exhibition | |
| Biomaterial Interfaces | Tuesday Sessions |
| Session BI+AS+BA+NL-TuM |
| Session: | Biointerface, Energy and Environmental Applications of QCM |
| Presenter: | S. Svedhem, Chalmers University of Technology, Sweden |
| Authors: | L. De Battice, Chalmers University of Technology, Sweden R. Frost, Chalmers University of Technology, Sweden A. Sundblom, AkzoNobel PPC, Sweden M. Persson, AkzoNobel PPC, Sweden M. Wallin, University of Gothenburg, Sweden J. Sturve, University of Gothenburg, Sweden S. Svedhem, Chalmers University of Technology, Sweden |
| Correspondent: | Click to Email |
To improve on the performance of silica-based nanomaterials, and to reduce environmental and health risks related to this development, it is important to learn about how engineered nanomaterials interact with e.g. biomolecules and biological barriers. We are also interested in the development of a generic screening methodology for nanoparticles, and to identify nanoparticle features which are likely to lead to effects in cells. The present results have been obtained with a set of five silica nanoparticles, four of which were spherical (about 20 nm in diameter) and one of which had an elongated shape (roughly 4 x 20 nm). Size and zeta potential measurements were performed, and the adsorption profiles for the nanoparticles when interacting with each of four model lipid membranes of different composition and net charge were monitored in real time using the quartz crystal microbalance with dissipation monitoring (QCM-D). We found clear differences in adsorption profiles on the model membranes with respect to surface coating, and particle shape. These results were compared to the results obtained when exposing frog cells to the same particles, using a conventional assay detecting cellular damage and cytotoxicity (through cell lactate dehydrogenase (LDH) release) and as well in experiments where the function of frog cells cultured on QCM-D sensors was studied by QCM-D (the method is published in Frost et al., Analytical Biochemistry, in press). In general, there were small effects on the cells.
The results will be discussed in the perspective of establishing (Q)SAR for nanoparticles.