AVS 60th International Symposium and Exhibition | |
Biomaterial Interfaces | Tuesday Sessions |
Session BI+AS+BA+NL-TuM |
Session: | Biointerface, Energy and Environmental Applications of QCM |
Presenter: | C.W. Frank, Stanford University |
Authors: | C.W. Frank, Stanford University M.E. Wiseman, DSM Research |
Correspondent: | Click to Email |
Adsorbed antibodies can take several orientations: end-on/fab-up, end-on/fab-down, side-on, and flat-on. Since the accessibility of antigens will depend on the antibody orientation, we have used QCM-D to monitor transient adsorption and have determined the orientation as a function of coverage. In addition, we have used simultaneous QCM-D and ellipsometry to distinguish between the “wet” mass consisting of protein plus coupled water and the “dry” mass consisting only of the protein. Finally, we have applied an alternative protocol for determining the state of hydration using only QCM-D. This involves a D2O exchange that allows determination of the dry mass. We conclude that the QCM-D signal of proteins in liquids contains a major component from coupled water.