| AVS 58th Annual International Symposium and Exhibition | |
| Biomaterial Interfaces Division | Monday Sessions |
| Session BI-MoA |
| Session: | Sensors and Fluidics for Biomedical Applications |
| Presenter: | Jhon Figueroa, University of South Florida |
| Authors: | J. Figueroa, University of South Florida S. Magana, University of South Florida D. Gomez, University of South Florida D.V. Lim, University of South Florida R. Schlaf, University of South Florida |
| Correspondent: | Click to Email |
Antibody films for the use in biosensors and assays are usually deposited via wet-chemical attachment methods. The presented experiments demonstrate that pneumatic spray deposition of antibody thin films from aqueous solution yields films of similar sensitivity and durability without special surface treatments and attachment chemistries. The experiments were performed using a commercially available low flow nebulizer in combination with a syringe pump and a substrate rotation stage to homogenize the coating.
In the experiments E. coli O157:H7 antibody was deposited on cleaned microscopy glass slides without any other pre-treatment. Standard wet-chemically prepared silanized glass slides using the avidin-biotin attachment scheme were also prepared for direct comparison of sensitivity and longevity of the spray based substrates. After incubation with GFP-labeled E. coli O157:H7 cells (ATCC 35150) ranging from 104-106 CFU/ml the slides were rinsed and AF647-labeled detector antibody was added and incubated. After rinsing and drying the slides were interrogated with a 635 nm laser and visualized using a CCD camera. Slides were also visualized by epifluorescence microscopy to examine antibody patterns and determine E. coli capture efficiencies.
The results of the experiments demonstrate that there is little difference between spray and standard protocol wet-chemically prepared substrates. This indicates that antibody films can be prepared via physisorption without complex attachment chemistries, and that antibodies can directly attach to glass slides, while retaining their functionality.