AVS 58th Annual International Symposium and Exhibition
    Biomaterial Interfaces Division Monday Sessions
       Session BI-MoA

Invited Paper BI-MoA1
Three-dimensional Microfluidic Flow Cell Array Integrated with SPR Microscopy for Multi-channel Bioassays

Monday, October 31, 2011, 2:00 pm, Room 108

Session: Sensors and Fluidics for Biomedical Applications
Presenter: Jennifer Shumaker-Perry, University of Utah
Correspondent: Click to Email
The expanding development and implementation of biotherapeutics to treat a wide range of diseases, including autoimmune diseases and cancer, have increased the need for immunogenicity assessment of these therapies. Immunogenicity is an immune system response of a patient to a drug. Anti-drug antibodies (ADAs) produced during the immune response may cause serious adverse effects such as reducing drug efficacy, altering pharmacokinetics, causing infusion reactions including anaphylaxis and serum sickness, and neutralizing native proteins. The complexity of immunogenicity analysis presents challenges in identifying and characterizing the neutralizing activity of ADAs. We have integrated a three-dimensional microfluidic flow cell array (MFCA) with surface plasmon resonance microscopy (SPRM) for a multi-channel, array-based approach to immunogenicity assessment. The microfluidic device provides 48 separate flow channels that can be used simultaneously for biomolecule immobilization and subsequent array-based biomolecule interaction analysis. Because the biomolecules can be immobilized in situ, exposure to harsh environments can be avoided, a major benefit for protein immobilization. In addition, the biomolecule immobilization process can be monitored in real time by SPR microscopy. This versatile, multi-channel biomolecule interaction analysis platform is being developed for ADA assessment which will benefit from the ability to implement controls, identify optimal assay conditions, and obtain detailed data about the nature of the biomolecule interactions. Characterization of the integrated system and initial investigations related to ADA assessment will be presented.