AVS 57th International Symposium & Exhibition
    Graphene Focus Topic Wednesday Sessions
       Session GR+EM+MS+TF+MI-WeM

Paper GR+EM+MS+TF+MI-WeM2
Graphene Oxide on Patterned Self-Assembly Monolayer for Cancer Marker Detection

Wednesday, October 20, 2010, 8:20 am, Room Brazos

Session: Graphene and Carbon-based Devices
Presenter: D.-J. Kim, Sungkyunkwan University, Republic of Korea
Authors: D.-J. Kim, Sungkyunkwan University, Republic of Korea
C.Y. Jung, Sungkyunkwan University, Republic of Korea
O.J. Yoon, Sungkyunkwan University, Republic of Korea
N.-E. Lee, Sungkyunkwan University, Republic of Korea
J.-S. Park, Korea Electronics Technology Institute, Republic of Korea
Correspondent: Click to Email
Graphene, two-dimensional sheet of sp2-hybridized carbon atom, has received much attention in recent years for many applications such as energy storage and conversion, batteries, fuel cells, optoelectronic device, field-effect transistors, electromechanical resonators, chemical sensors, and biotechnologies. Oxidized form of graphene, graphene oxide, which has functional groups of epoxide, carbonyls, hydroxide, and phenol groups at both sides can also provide various functionalities for biomedical applications because the high densities of defective sites can provide many active sites for electron transfer to biological species, similarly to graphene. In this study, we demonstrate the graphene oxide based immunosensor for cancer marker detection. Graphene oxide pattern was formed selectively on self-assembly monolayer (SAM) pattern formed by using inkjet printing. The hydroxyl terminated ITO electrode was first selectively functionalized by the amine (-NH2) group by inkjet printing of SAM, and the graphene oxide flakes were adsorbed selectively on the SAM pattern. After immobilization of monoclonal antibody to PSA (prostate specific antigen) which is the biomarker in prostate cancer, the electrochemical performance of immunosensor was evaluated by varying the concentration of PSA-ACT (prostate specific antigen/α1-antichymotrypsin) complex. And the PSA polyclonal antibody conjugated with gold nanoparticles was applied to amplification of electrochemical signal because the attachment of nanoparticles onto electrodes drastically enhances the conductivity and electron transfer from the redox analytes. The results showed that graphene oxide based immunosensor can be possibly applied to the delicate diagnosis of various disease markers as well as PSA at the extremely low level and with ultra-high sensitivity.