AVS 57th International Symposium & Exhibition
    Biomaterial Interfaces Friday Sessions
       Session BI+MN-FrM

Paper BI+MN-FrM2
Enzymatic Activity Enhancement on Nano-structured TiO2 Platforms by Ru doping for Biosensor Applications

Friday, October 22, 2010, 8:40 am, Room Taos

Session: Sensors & Fluidics for Biomedical Applications
Presenter: R.R. Pandey, Centre for Cellular and Molecular Biology, India
Authors: R.R. Pandey, Centre for Cellular and Molecular Biology, India
K.K. Saini, National Physical Laboratory, India
M. Dhayal, Centre for Cellular and Molecular Biology, India
Correspondent: Click to Email
Devices based on nanomaterials platforms are emerging as a powerful tool for ultrasensitive sensors for the direct detection of biological and chemical species. In this work, we will report the preparation and the full characterization of Ru doped TiO2 nanostructured platforms those have been used for electrochemical detection of enzymatic activity for biosensor applications. Ru doped TiO2 platforms were prepared by sol-gel deposition onto conducting substrates and as test model cholesterol oxidase, urease and gucose oxidase were loaded onto the nanostructured platforms. Cholesterol /Urease/Glucose oxidase immobilized onto Ru doped TiO2-based nanostructured surfaces exhibited a pair of well-defined and quasireversible voltammetric peaks in CV measurements. The electron exchange between the enzyme and the electrodes was greatly enhanced in the Ru doped TiO2 nanostructured environment. The electrocatalytic activity of cholesterol, uresae and glucose oxidase embedded on Ru doped TiO2 electrodes had enhanced significantly toward detection of low concentrations of cholesterol, urea and glucose.