AVS 56th International Symposium & Exhibition
    Biomaterial Interfaces Thursday Sessions
       Session BI-ThP

Paper BI-ThP3
High Sensitivity Electrochemcial Immunosensor Based on Plasma Modified TiO2/Chitosan

Thursday, November 12, 2009, 6:00 pm, Room Hall 3

Session: Biomaterial Interfaces Poster Session II (Arrays, Sensing, Micro/Nanofabrication, SPM)
Presenter: M. Dhayal, Centre for Cellular and Molecular Biology, India
Authors: R. Khan, North East Institute of Science & Technology, India
M. Dhayal, Centre for Cellular and Molecular Biology, India
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RF plasma treated nanocrystalline hybrid chitosan/TiO2 matrix was used to develop high sensitivity electrochemical immnunosensor. The observed interfacial charge transfer resistance (RCT) and double layer capacitance (Cd) of plasma modified matrixes were decreased. An improved strength of amide I and II groups in FTIR spectra were observed which was associated with enhancement in immobilized rabbit antibodies (IgGs) on plasma altered surfaces. Electrochemically q uantitative detection of Ochratoxin-A (OTA) concentrations varying in buffer solution was carried out on IgGs immobilized plasma treated and untreated ITO/CS/TiO2 electrodes. Plasma modified electrodes had showed very good sensitivity at very low OTA concentrations whereas the untreated electrodes sensitivity was deprived.