| AVS 56th International Symposium & Exhibition | |
| Biomaterial Interfaces | Wednesday Sessions |
| Session BI+AS+NS-WeA |
| Session: | Quantitative Nanoscale Sensing at Biosurfaces and Interfaces |
| Presenter: | D.W. Moon, Korea Research Institute of Standards and Science, S. Korea |
| Authors: | D.W. Moon, Korea Research Institute of Standards and Science, S. Korea J. Gil, Korea Research Institute of Standards and Science, S. Korea W. CheGal, Korea Research Institute of Standards and Science, S. Korea H. Cho, Korea Research Institute of Standards and Science, S. Korea S. Kim, Korea Research Institute of Standards and Science, S. Korea |
| Correspondent: | Click to Email |
The interaction between cell and extracellular matrix (ECM) governs multiple cellular functions and contributes to promote inflammation and tumor metastasis. Therefore, cellular behavior needs to be monitored in the ECM interactive circumstance. Most of previous studies on cell adhesion are based on immunofluorescence microscopy. For cell adhesion dynamics studies, label-free optical techniques that can monitor continuously cell-ECM interfaces for living cells are required.
Here we developed surface plasmon resonance imaging ellipsometry (SPRIE) which can simply image cell-ECM interfaces for live cells with high contrast and at real-time. To visualize cell adhesions to ECM, null-type imaging ellipsometry technique with the attenuated total reflection coupler was applied and both of transverse magnetic and electric waves were made use of. These characteristics make it possible to acquire the high contrast image of cell adhesions. Different features and dynamics of cell adhesion patters in ~ 100 nm cell-ECM interfaces were observed for A10, human coronary artery smooth muscle cell hCASMC, and human umbilical vein endothelial cells (HUVEC) on fibronectin and collagen ECM layers with 1 μm spatial resolution and 30 sec time interval upto 3 days. Harmonized changes of entire adhesion proteins were observed during cell division and cell migration through our imaging system without any labeling. SPRIE images were compared with confocal fluorescence microscopic images of cell adhesion proteins for validation of SPRIE images. Preliminary results on SPRIE studies on the effect of shear force on cell adhesion and migration will be also discussed.
We expect that SPRIE cell adhesion dynamic imaging methods would be useful for further understanding of cell biology and development of drug screening methodology relevant to cell adhesion and migration.