|AVS 56th International Symposium & Exhibition|
|Biomaterial Interfaces||Friday Sessions|
|Session:||Micro and Nanoengineering of Biointerfaces II|
|Presenter:||M. Palma, Columbia University|
|Authors:||M. Palma, Columbia University
J. Abramson, Columbia University
M. Schvartzmann, Columbia University
A. Gorodetsky, Columbia University
C. Nuckolls, Columbia University
M.P. Sheetz, Columbia University
J. Hone, Columbia University
S.J. Wind, Columbia University
|Correspondent:||Click to Email|
Combining nanolithography and biomolecular self-assembly strategies, we report on the fabrication of nanopatterned biomimetic surfaces and their use in a variety of biological studies.
We have fabricated arrays of Au/Pd nano-dots of dimensions down to the sub-10nm regime using electron-beam and nanoimprint lithography. Different chemical strategies at surfaces have been pursued to organize biological relevant nanoarchitectures into hierarchical arrays in which structural parameters, such as the spacing and nature of specific functional groups, could be systematically varied and controlled.
The generation of DNA nano-dot arrays allowed us to follow the activity (at surfaces) of a restriction enzyme in real time and at the nanoscale: fluorescence microscopy enabled the monitoring of the kinetics of such protein-DNA interaction.
Furthermore we will show how our nanopatterned biomimetic surfaces can be used to probe the importance of both the geometric arrangement (i.e. spatial ordering of transmembrane proteins, integrins) as well as the role played by peptide sequences as cell binding domains in the formation of cell focal adhesions.
Finally, we will highlight the broader utility and application of such functional nanopatterned surfaces for nanoscopic control and studies: biochemical specificity can be used to selectively place individual nanocomponents with a high degree of control over both position and orientation, as well as to organize functional nanostructures into dense arrays with very fine pitch.